7DIE
Crystal structure of M. penetrans Ferritin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-07-17 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.979 |
Spacegroup name | I 4 |
Unit cell lengths | 151.334, 151.334, 125.213 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.856 - 1.900 |
R-factor | 0.1536 |
Rwork | 0.153 |
R-free | 0.17720 |
Structure solution method | SAD |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | AutoSol |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmeas | 0.108 | 0.463 |
Rpim | 0.041 | 0.174 |
Number of reflections | 110615 | 5458 |
<I/σ(I)> | 30.79 | 3.875 |
Completeness [%] | 99.7 | |
Redundancy | 6.9 | |
CC(1/2) | 0.992 | 0.927 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 0.2 M ammonium acetate, 0.1 M Tris-HCl (pH 8.5), 10-15% (w/v) MPD |