7DHY
Arsenic-bound p53 DNA-binding domain mutant G245S
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-01-11 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.109, 68.243, 83.621 |
| Unit cell angles | 90.00, 90.03, 90.00 |
Refinement procedure
| Resolution | 48.210 - 2.150 |
| R-factor | 0.1888 |
| Rwork | 0.187 |
| R-free | 0.21900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2j1y |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.304 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.220 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.083 | 0.260 |
| Number of reflections | 41923 | 3334 |
| <I/σ(I)> | 12.7 | 6.6 |
| Completeness [%] | 94.8 | 93.3 |
| Redundancy | 4.2 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M Tris (pH 8.0-8.5), 20% (w/v) PEG 8000, 0.2 M Li2SO4, 2 mM EDTA, and 2 mM As2O3 |
