7DFC
Crystal of Arrestin2-V2Rpp-3-Fab30 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-06-29 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0500 |
Spacegroup name | I 21 21 21 |
Unit cell lengths | 116.137, 122.653, 144.064 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.690 - 2.490 |
R-factor | 0.2315 |
Rwork | 0.229 |
R-free | 0.28400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4jqi |
RMSD bond length | 0.009 |
RMSD bond angle | 1.159 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.690 | 2.550 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.062 | 0.959 |
Rpim | 0.025 | 0.548 |
Number of reflections | 38166 | 4165 |
<I/σ(I)> | 18.5 | |
Completeness [%] | 99.6 | |
Redundancy | 7.2 | |
CC(1/2) | 0.999 | 0.850 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 7.5 | 289 | 15% PEG 3350, 0.1M HEPES, 0.2mM DMSO |