7DEI

Structure of human ORP3 ORD domain in complex with PI(4)P

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-27
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97949
Spacegroup name	P 31 2 1
Unit cell lengths	95.801, 95.801, 190.456
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.450 - 2.600
R-factor	0.2234
Rwork	0.220
R-free	0.26880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4inq
RMSD bond length	0.011
RMSD bond angle	1.612
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.640
High resolution limit [Å]	2.600	2.600
Rmerge	0.127	0.530
Number of reflections	31618	1364
<I/σ(I)>	39	4.4
Completeness [%]	99.0	88.3
Redundancy	6.2	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	295	0.1 M MES-NaOH pH 6.0, 25% PEG 1500, 0.1 M Magnesium chloride