7DE1
Crystal structure of SARS-CoV-2 nucleocapsid protein C-terminal RNA binding domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2020-03-07 |
Detector | OXFORD RUBY CCD |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 45.214, 101.438, 59.526 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 18.480 - 2.000 |
R-factor | 0.1765 |
Rwork | 0.171 |
R-free | 0.22270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2cjr |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 18.790 | 2.071 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.059 | 0.213 |
Number of reflections | 19057 | 1805 |
<I/σ(I)> | 18.58 | |
Completeness [%] | 99.3 | |
Redundancy | 3.6 | |
CC(1/2) | 0.997 | 0.921 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.3 | 289.15 | 100 mM CHES (pH 9.3) , 40% PEG 6000 |