7DE1

Crystal structure of SARS-CoV-2 nucleocapsid protein C-terminal RNA binding domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	CCD
Collection date	2020-03-07
Detector	OXFORD RUBY CCD
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	45.214, 101.438, 59.526
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.480 - 2.000
R-factor	0.1765
Rwork	0.171
R-free	0.22270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2cjr
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	18.790	2.071
High resolution limit [Å]	2.000	2.000
Rmerge	0.059	0.213
Number of reflections	19057	1805
<I/σ(I)>	18.58
Completeness [%]	99.3
Redundancy	3.6
CC(1/2)	0.997	0.921

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9.3	289.15	100 mM CHES (pH 9.3) , 40% PEG 6000