7DCU
Crystal structure of HSF2 DNA-binding domain in complex with 3-site HSE DNA (21 bp)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2017-07-07 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9774 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 46.640, 67.060, 139.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 69.825 - 1.750 |
| R-factor | 0.1738 |
| Rwork | 0.172 |
| R-free | 0.20460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5hdk |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.969 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.830 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rpim | 0.033 | 0.401 |
| Number of reflections | 44148 | |
| <I/σ(I)> | 13.5 | |
| Completeness [%] | 98.0 | |
| Redundancy | 10.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M HEPES pH 7.5 and 25% (w/v) PEG 3350 |
