7DCD
Nonstructural protein 7 and 8 complex of SARS-CoV-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2020-04-18 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97930 |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.031, 42.990, 100.630 |
| Unit cell angles | 90.01, 90.03, 72.61 |
Refinement procedure
| Resolution | 41.065 - 2.570 |
| R-factor | 0.2582 |
| Rwork | 0.254 |
| R-free | 0.35330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ahm |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.239 |
| Data scaling software | HKL-2000 |
| Refinement software | PHENIX (Phenix1.18-3845) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.670 |
| High resolution limit [Å] | 2.570 | 2.590 |
| Rmerge | 0.200 | |
| Rpim | 0.066 | |
| Number of reflections | 20420 | 1776 |
| <I/σ(I)> | 8.5 | |
| Completeness [%] | 93.2 | |
| Redundancy | 3.3 | |
| CC(1/2) | 0.942 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 293 | 200mM sodium chloride, 100mM Tris-HCl, pH 8.2, 25% PEG 3350 |
