7DBG

Yeast CRM1e (apo) in complex with Ran-RanBP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2020-01-01
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	P 43 21 2
Unit cell lengths	105.900, 105.900, 304.290
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.120 - 2.060
R-factor	0.2023
Rwork	0.201
R-free	0.22840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6m6x
RMSD bond length	0.004
RMSD bond angle	1.273
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.120	2.110
High resolution limit [Å]	2.060	2.060
Number of reflections	107784	7866
<I/σ(I)>	15.5
Completeness [%]	100.0
Redundancy	25.2
CC(1/2)	0.999	0.710

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296	0.12 M Monosaccharides (20 mM D-Glucose; 20 mM D-Mannose; 20 mM D-Galactose; 20 mM L-Fucose; 20 mM D-Xylose; 20 mM N-Acetyl-D-Glucosamine), 0.1 M buffer system 1 pH 6.5 (sodium HEPES and MOPS), and 50 % Precipitant Mix 2 (40% v/v Ethylene glycol; 20 % w/v PEG 8000)