7DAE

EPB in complex with tubulin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9785
Spacegroup name	P 21 21 21
Unit cell lengths	104.792, 157.210, 181.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.713 - 2.394
R-factor	0.1752
Rwork	0.174
R-free	0.22150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4i55
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.440
High resolution limit [Å]	2.394	6.510	2.400
Rmerge	0.157	0.051	1.115
Rmeas	0.164	0.053	1.180
Rpim	0.045	0.015	0.378
Number of reflections	118328	6277	5839
<I/σ(I)>	3.9
Completeness [%]	99.9	99.8	99.2
Redundancy	13.1	12.5	9.3
CC(1/2)		0.999	0.644

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.7	277	6% PEG, 5% glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCL2, pH 6.7