7D9V
CRTC1 pSer245 peptide in complex with 14-3-3 zeta
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-10-31 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.2782 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 70.975, 83.158, 110.831 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.110 - 2.210 |
R-factor | 0.208 |
Rwork | 0.206 |
R-free | 0.23970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1a4o |
Data reduction software | AUTOMAR |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.240 |
High resolution limit [Å] | 2.200 | 5.970 | 2.200 |
Rmerge | 0.117 | 0.041 | 0.795 |
Rmeas | 0.129 | 0.045 | 0.879 |
Rpim | 0.053 | 0.019 | 0.368 |
Total number of observations | 207559 | ||
Number of reflections | 36010 | 1993 | 1601 |
<I/σ(I)> | 4 | ||
Completeness [%] | 98.4 | 99.7 | 88.4 |
Redundancy | 5.8 | 5.7 | 4.8 |
CC(1/2) | 0.998 | 0.896 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 200mM Ammonium acetate, 100mM Bis-Tris (pH6.5), 25% (w/v) Polyethylene glycol 3350 |