7D8B
Engineering Disulphide-Free Autonomous Antibody VH Domains to modulate intracellular pathways
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-04 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.953700 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.595, 140.394, 61.961 |
| Unit cell angles | 90.00, 104.04, 90.00 |
Refinement procedure
| Resolution | 46.216 - 2.460 |
| Rwork | 0.250 |
| R-free | 0.29180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v8w |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.185 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.197 | 2.590 |
| High resolution limit [Å] | 2.460 | 2.460 |
| Number of reflections | 28611 | 4143 |
| <I/σ(I)> | 3.6 | 0.9 |
| Completeness [%] | 99.9 | |
| Redundancy | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.01M Tri-sodium citrate, 16% PEG 6000 |
