7D87

Crystal Structure of zebrafish PHF14-PZP in complex with H3(1-25)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	C 2 2 21
Unit cell lengths	41.921, 151.019, 81.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.210 - 2.110
R-factor	0.167
Rwork	0.165
R-free	0.20430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7d86
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.140
High resolution limit [Å]	2.100	5.700	2.100
Rmerge	0.125	0.061	0.889
Rmeas	0.130	0.064	0.943
Rpim	0.037	0.019	0.306
Total number of observations	181303
Number of reflections	15363	850	748
<I/σ(I)>	7
Completeness [%]	99.8	98.8	99.9
Redundancy	11.8	10.7	8.7
CC(1/2)		0.998	0.854

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	291	0.1 M Carboxylic acids,0.1 M Buffer System 2, 37.50% % v/v MPD_P1K_P3350 (morpheus).