7D6F
The crystal structure of ARMS-PBM/MAGI2-PDZ4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-05-08 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97776 |
Spacegroup name | C 2 2 2 |
Unit cell lengths | 74.045, 95.666, 35.167 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.277 - 3.001 |
R-factor | 0.2381 |
Rwork | 0.235 |
R-free | 0.28140 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5zys |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP (3.25) |
Refinement software | PHENIX (v1.13) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.750 |
High resolution limit [Å] | 2.700 | 7.320 | 2.700 |
Rmerge | 0.092 | 0.047 | 0.724 |
Rmeas | 0.096 | 0.049 | 0.757 |
Rpim | 0.027 | 0.014 | 0.219 |
Total number of observations | 46340 | ||
Number of reflections | 3696 | 218 | 178 |
<I/σ(I)> | 4.9 | ||
Completeness [%] | 100.0 | 99.5 | 100 |
Redundancy | 12.5 | 10.5 | 11.8 |
CC(1/2) | 0.999 | 0.967 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1 M HEPES pH 7.5, 10% w/v Polyethylene glycol 8000, 8% v/v Ethylene glycol |