7D3D

Crystal structure of SPOP bound with a peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9735
Spacegroup name	P 21 21 21
Unit cell lengths	51.196, 58.555, 94.180
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.450
R-factor	0.1639
Rwork	0.163
R-free	0.18900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ivv
RMSD bond length	0.009
RMSD bond angle	1.404
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.730	30.000	1.500
High resolution limit [Å]	1.440	3.120	1.450
Rmerge	0.073	0.053	0.474
Rmeas	0.076	0.056	0.512
Rpim	0.021	0.016	0.189
Number of reflections	50483	5440	4277
<I/σ(I)>	8.4
Completeness [%]	98.1	100	84.4
Redundancy	11.9	12.6	6.9
CC(1/2)		0.999	0.897

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	200 mM sodium acetate trihydrate, pH 7.0, 20%(w/v) polyethylene glycol 3350