7D2T

Crystal structure of Rsu1/PINCH1_LIM45C complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-04-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	114.624, 51.293, 119.626
Unit cell angles	90.00, 101.55, 90.00

Refinement procedure

Resolution	50.000 - 2.200
R-factor	0.1709
Rwork	0.170
R-free	0.19780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7d2s
RMSD bond length	0.003
RMSD bond angle	0.735
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.240
High resolution limit [Å]	2.200	5.970	2.200
Rmerge	0.152	0.100	1.204
Rmeas	0.165	0.109	1.301
Rpim	0.064	0.043	0.490
Number of reflections	70288	3706	3504
<I/σ(I)>	4.8
Completeness [%]	100.0	99.9	100
Redundancy	6.7	6.3	6.9
CC(1/2)		0.992	0.687

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.1 M sodium malonate pH7.0, 12% w/v Polyethylene glycol 3350