7D0V
Crystal structure of Taiwan cobra 5'-nucleotidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-07-19 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.9998 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.327, 68.127, 140.214 |
| Unit cell angles | 90.00, 95.17, 90.00 |
Refinement procedure
| Resolution | 28.670 - 2.175 |
| R-factor | 0.1775 |
| R-free | 0.21860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4h1s |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.858 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16-3549-000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.260 |
| High resolution limit [Å] | 2.175 | 2.175 |
| Rmerge | 0.117 | 0.588 |
| Number of reflections | 52203 | 4804 |
| <I/σ(I)> | 10.96 | |
| Completeness [%] | 99.9 | |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 293 | 25% PEG 3350, 0.2M Magnesium chloride hexahydrate, 0.1 M Bis-Tris pH 6.7 |
