7D0N

Crystal structure of mouse CRY2 apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	P 43
Unit cell lengths	67.840, 67.840, 128.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.920 - 2.800
R-factor	0.2526
Rwork	0.251
R-free	0.29340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6kx8
RMSD bond length	0.006
RMSD bond angle	0.783
Data reduction software	MOSFLM (7.3.0)
Data scaling software	SCALA (3.3.22)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.120	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.135	0.604
Rmeas	0.147	0.662
Rpim	0.058	0.267
Number of reflections	14322	2112
<I/σ(I)>	10.9	2.9
Completeness [%]	99.9	100
Redundancy	6.3
CC(1/2)	0.995	0.746

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.1 M HEPES pH 7.0, 100 mM NaCl, 10% w/v PEG 3350