7CZM
Crystal structure of FIP200 Claw/p-OPtineurin LIR complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-05-03 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97918 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 73.978, 70.624, 56.337 |
Unit cell angles | 90.00, 109.27, 90.00 |
Refinement procedure
Resolution | 34.960 - 2.000 |
Rwork | 0.203 |
R-free | 0.22340 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6dce |
RMSD bond length | 0.007 |
RMSD bond angle | 0.865 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.320 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.034 | 0.254 |
Number of reflections | 18560 | 1836 |
<I/σ(I)> | 31.6 | |
Completeness [%] | 99.6 | |
Redundancy | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | Potassium iodide, MES pH 6.5, PEG 4000 |