7CZF

Crystal structure of Kaposi Sarcoma associated herpesvirus (KSHV ) gHgL in complex with the ligand binding domian (LBD) of EphA2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2019-05-23
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97919
Spacegroup name	P 21 21 21
Unit cell lengths	80.803, 153.196, 275.228
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.650 - 3.200
R-factor	0.2409
Rwork	0.239
R-free	0.27210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5w0k 3fl7
RMSD bond length	0.002
RMSD bond angle	0.477
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.310
High resolution limit [Å]	3.200	3.200
Number of reflections	57447	5657
<I/σ(I)>	18.3
Completeness [%]	99.6
Redundancy	12.9
CC(1/2)	0.997	0.527

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.25 M Potassium phosphate dibasic, 17% w/v Polyethylene glycol 3,350