7CZE

Crystal structure of Epstein-Barr virus (EBV) gHgL and in complex with the ligand binding domian (LBD) of EphA2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-14
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97853
Spacegroup name	P 1
Unit cell lengths	94.464, 113.806, 119.654
Unit cell angles	90.03, 90.17, 89.89

Refinement procedure

Resolution	41.220 - 3.000
R-factor	0.2587
Rwork	0.257
R-free	0.28500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5w0k 3fl7
RMSD bond length	0.005
RMSD bond angle	1.010
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.110
High resolution limit [Å]	3.000	3.000
Number of reflections	98531	9508
<I/σ(I)>	12.95
Completeness [%]	99.0
Redundancy	3.5
CC(1/2)	0.994	0.757

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	1% w/v Tryptone, 0.001 M Sodium azide, 0.05 M HEPES sodium pH 7.0, 12% w/v Polyethylene glycol 3,350