7CYU
Crystal structure of human BAF57 HMG domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2019-03-25 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 58.873, 58.873, 50.263 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.790 - 2.550 |
R-factor | 0.2377 |
Rwork | 0.233 |
R-free | 0.27480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3tq6 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.471 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.590 |
High resolution limit [Å] | 2.550 | 2.550 |
Number of reflections | 3481 | 312 |
<I/σ(I)> | 21.62 | 1.88 |
Completeness [%] | 98.9 | 95.2 |
Redundancy | 7 | |
CC(1/2) | 0.998 | 0.844 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 290 | 2.0M Ammonium sulfate, 0.1M Sodium acetate |