7CYM

Crystal structure of LI-Cadherin EC1-4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.00000
Spacegroup name	P 1 21 1
Unit cell lengths	80.360, 70.840, 134.219
Unit cell angles	90.00, 98.69, 90.00

Refinement procedure

Resolution	55.250 - 2.700
R-factor	0.2247
Rwork	0.222
R-free	0.27440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zmy
RMSD bond length	0.013
RMSD bond angle	1.835
Data reduction software	MOSFLM (7.2.2)
Data scaling software	SCALA (3.3.22)
Phasing software	PHASER (2.8.2)
Refinement software	REFMAC (5.8.0230)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.200	2.850
High resolution limit [Å]	2.700	2.700
Rmerge	0.095	0.858
Rpim	0.041	0.371
Number of reflections	41272	5955
<I/σ(I)>	1.8	1.8
Completeness [%]	99.9	100
Redundancy	6.1	6.2
CC(1/2)	0.998	0.907

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293.15	200 mM sodium sulfate, 20% w/v Polyethylene glycol 3350