7CYL

Crystal structure of Karyopherin-beta2 in complex with FUS PY-NLS(P525L)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-19
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97915
Spacegroup name	P 21 21 2
Unit cell lengths	129.190, 158.366, 68.712
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.060 - 2.700
R-factor	0.198
Rwork	0.196
R-free	0.22840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fdd
RMSD bond length	0.010
RMSD bond angle	1.457
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.060	2.800
High resolution limit [Å]	2.700	2.700
Number of reflections	39428	3860
<I/σ(I)>	19.3	2.2
Completeness [%]	99.7
Redundancy	7
CC(1/2)	0.999	0.815

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1 M Succinic acid (pH7.0), 1%(w/v) PEG MME2000