7CY8
Crystal Structure of CMD1 in complex with 5mC-DNA and vitamin C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NFPSS BEAMLINE BL18U |
| Synchrotron site | NFPSS |
| Beamline | BL18U |
| Temperature [K] | 173 |
| Detector technology | PIXEL |
| Collection date | 2020-05-17 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 154.488, 125.646, 64.058 |
| Unit cell angles | 90.00, 102.96, 90.00 |
Refinement procedure
| Resolution | 49.280 - 2.400 |
| R-factor | 0.1923 |
| Rwork | 0.190 |
| R-free | 0.23510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7cy4 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.281 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.280 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.088 | 1.020 |
| Number of reflections | 35155 | 1758 |
| <I/σ(I)> | 13.1 | 1.6 |
| Completeness [%] | 94.6 | |
| Redundancy | 4.7 | |
| CC(1/2) | 1.000 | 0.530 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 290 | 15% (v/v) 2-propanol, 0.1 M sodium citrate, pH 5.0, and 10% (w/v) PEG 10000 |
