7CY4
Crystal Structure of CMD1 in apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-10 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 154.677, 123.573, 64.206 |
| Unit cell angles | 90.00, 103.16, 90.00 |
Refinement procedure
| Resolution | 47.770 - 2.200 |
| R-factor | 0.1815 |
| Rwork | 0.180 |
| R-free | 0.22310 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.241 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.770 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.073 | 1.240 |
| Number of reflections | 48745 | 2437 |
| <I/σ(I)> | 17.2 | 1.7 |
| Completeness [%] | 94.3 | 91.6 |
| Redundancy | 6.9 | 6.6 |
| CC(1/2) | 1.000 | 0.560 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 290 | 2% (v/v) tacsimate, pH 5.0, 0.1 M sodium citrate, pH 5.6, 16% (w/v) PEG 3350 |
