7CWD
Crystal structure of beta-galactosidase II from Bacillus circulans in complex with beta-D-galactopyranosyl disaccharide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-11-04 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.97934 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 159.499, 159.499, 96.326 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 33.280 - 2.000 |
Rwork | 0.189 |
R-free | 0.21800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ypj |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (v5.0) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.030 |
High resolution limit [Å] | 2.000 | 5.430 | 2.000 |
Rmerge | 0.082 | 0.054 | 0.297 |
Rmeas | 0.090 | 0.058 | 0.333 |
Rpim | 0.036 | 0.022 | 0.146 |
Total number of observations | 497629 | ||
Number of reflections | 89811 | 4787 | 4620 |
<I/σ(I)> | 11.4 | ||
Completeness [%] | 94.8 | 96.4 | 99.4 |
Redundancy | 5.5 | 6.8 | 4.8 |
CC(1/2) | 0.997 | 0.940 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | Ammonium sulfate, Sodium acetate, Sodium chloride |