7CVS
Crystal structure of the C85A/L194A mutant CLC-ec1 with Fab fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2017-03-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 232.909, 96.721, 170.731 |
| Unit cell angles | 90.00, 131.65, 90.00 |
Refinement procedure
| Resolution | 33.960 - 3.010 |
| R-factor | 0.2031 |
| Rwork | 0.201 |
| R-free | 0.25510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ene |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.707 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.960 | 3.050 |
| High resolution limit [Å] | 3.005 | 3.005 |
| Rmerge | 0.173 | 1.842 |
| Rpim | 0.082 | 0.840 |
| Number of reflections | 54754 | 2148 |
| <I/σ(I)> | 24.1 | 1.52 |
| Completeness [%] | 96.5 | 87.7 |
| Redundancy | 5.4 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 29% (w/v) PEG400 100 mM Hepes-NaOH (pH 7.0) 200 mM NaCl |
