7CVP
The Crystal Structure of human PHGDH from Biortus.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-26 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.101, 121.638, 59.372 |
| Unit cell angles | 90.00, 99.97, 90.00 |
Refinement procedure
| Resolution | 42.486 - 2.500 |
| Rwork | 0.261 |
| R-free | 0.31960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6cwa |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.326 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.486 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.064 | 0.130 |
| Number of reflections | 19518 | 2202 |
| <I/σ(I)> | 6.4 | 2.2 |
| Completeness [%] | 93.7 | 94.5 |
| Redundancy | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M Bis-Tris pH6.5, 25% PEG3,350 |
