7CVH

Human Fructose-1,6-bisphosphatase 1 in complex with geranylgeranyl diphosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-01
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	0.97853
Spacegroup name	P 21 21 21
Unit cell lengths	67.392, 83.426, 277.355
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.250 - 2.090
R-factor	0.1789
Rwork	0.178
R-free	0.21830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vt5
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.140
High resolution limit [Å]	2.090	5.700	2.100
Rmerge	0.366	0.078	1.019
Rmeas	0.413	0.088	1.240
Rpim	0.187	0.040	0.692
Number of reflections	89144	4767	4257
<I/σ(I)>	3.9
Completeness [%]	95.8	95	93.5
Redundancy	3.7	3.8	2.5
CC(1/2)		0.997	0.352

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	28% (v/v) polyethylene glycol 600, 0.1M HEPES