7CSW

Pseudomonas aeruginosa antitoxin HigA with pa2440 promoter

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97853
Spacegroup name	P 32
Unit cell lengths	58.498, 58.498, 66.795
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.360 - 1.970
R-factor	0.201
Rwork	0.197
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6f8h
RMSD bond length	0.008
RMSD bond angle	0.892
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.360	2.040
High resolution limit [Å]	1.969	1.970
Rmerge	0.169
Number of reflections	18109
<I/σ(I)>	14.4
Completeness [%]	99.9
Redundancy	9.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		290	1M KCl, 0.1M Na-HEPES pH7.5, 1M (NH4)2SO4