7CSV
Pseudomonas aeruginosa antitoxin HigA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-08-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97930 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 65.689, 65.689, 140.392 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.540 - 1.710 |
| R-factor | 0.1833 |
| Rwork | 0.179 |
| R-free | 0.22260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6f8h |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.808 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.540 | 1.768 |
| High resolution limit [Å] | 1.707 | 1.707 |
| Rmerge | 0.116 | |
| Number of reflections | 33999 | 1875 |
| <I/σ(I)> | 6.4 | |
| Completeness [%] | 99.9 | |
| Redundancy | 33.8 | |
| CC(1/2) | 0.996 | 0.993 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 290 | 0.2M MgCl2, 0.1M Tris pH 8.5, 20% PEG8000 |
