7CR5

Complex structure of a human monoclonal antibody with SARS-CoV-2 nucleocapsid protein NTD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-05-16
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 2
Unit cell lengths	154.065, 52.596, 85.299
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.580 - 2.080
R-factor	0.1923
Rwork	0.191
R-free	0.22200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6m3m 6tcn
RMSD bond length	0.008
RMSD bond angle	0.876
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.140
High resolution limit [Å]	2.080	2.100
Rmerge	0.220	1.331
Rmeas	0.230	1.405
Rpim	0.064
Number of reflections	41759	2023
<I/σ(I)>	13.18	1.64
Completeness [%]	99.9	99.6
Redundancy	12.1	8.4
CC(1/2)	0.979	0.782

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.2	289.15	0.01M Calcium chloride dihydrate, 0.05 M Sodium cacodylate trihydrate (pH 7.2) ,1.675M Ammonium sulfate, 0.5mM spermine