7CQZ
crystal structure of mouse FAM46C (TENT5C)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-05 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.827, 59.200, 173.432 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.347 - 2.350 |
| R-factor | 0.220033268159 |
| Rwork | 0.217 |
| R-free | 0.24633 |
| Structure solution method | SAD |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.378 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.360 | 2.480 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.055 | 0.572 |
| Rmeas | 0.061 | 0.626 |
| Rpim | 0.025 | 0.252 |
| Number of reflections | 22719 | 3281 |
| <I/σ(I)> | 17.8 | 3.1 |
| Completeness [%] | 98.7 | 99 |
| Redundancy | 5.9 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2 M sodium citrate tribasic pH 6.5, 16 % w/v PEG3350 |
