7CPZ
Crystal structure of Streptoavidin-C1 from Streptomyces cinamonensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 11C |
| Synchrotron site | PAL/PLS |
| Beamline | 11C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-05-08 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 57.997, 57.997, 74.847 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.420 - 2.500 |
| R-factor | 0.2085 |
| Rwork | 0.206 |
| R-free | 0.26110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bc3 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.856 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2-3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmeas | 0.335 | |
| Rpim | 0.090 | 0.258 |
| Number of reflections | 4763 | 229 |
| <I/σ(I)> | 7.51 | |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 20 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 298 | PEG3350 |
