2BC3

T7-tagged full-length streptavidin

Summary for 2BC3

• Entry DOI: 10.2210/pdb2bc3/pdb
• Descriptor: Streptavidin, GLYCEROL, SULFATE ION, ... (4 entities in total)
• Functional Keywords: streptavidin, t7 tag, biotin binding protein
• Biological source: Streptomyces avidinii
• Cellular location: Secreted: P22629
• Total number of polymer chains: 2
• Total formula weight: 33420.29

Authors

Stenkamp, R.E.,Le Trong, I.,Ward, T.R.,Humbert, N. (deposition date: 2005-10-18, release date: 2005-10-25, Last modification date: 2024-04-03)

Primary citation

Le Trong, I.,Humbert, N.,Ward, T.R.,Stenkamp, R.E.
Crystallographic Analysis of a Full-length Streptavidin with Its C-terminal Polypeptide Bound in the Biotin Binding Site.
J.Mol.Biol., 356:738-745, 2006

PubMed Abstract: The structure of a full-length streptavidin has been determined at 1.7 A resolution and shows that the 20 residue extension at the C terminus forms a well-ordered polypeptide loop on the surface of the tetramer. Residues 150-153 of the extension are bound to the ligand-binding site, possibly competing with exogenous ligands. The binding mode of these residues is compared with that of biotin and peptidic ligands. The observed structure helps to rationalize the observations that full-length mature streptavidin binds biotinylated macromolecules with reduced affinity.

PubMed: 16384581
DOI: 10.1016/j.jmb.2005.11.086

Experimental method

X-RAY DIFFRACTION (1.54 Å)