7CPR
glutamine synthetase from Drosophila
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-10-23 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 229.698, 102.919, 206.508 |
| Unit cell angles | 90.00, 120.09, 90.00 |
Refinement procedure
| Resolution | 178.680 - 2.120 |
| R-factor | 0.2001 |
| Rwork | 0.198 |
| R-free | 0.24570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ojw |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.732 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 178.680 | 2.200 |
| High resolution limit [Å] | 2.120 | 2.120 |
| Number of reflections | 234758 | 234758 |
| <I/σ(I)> | 16.14 | |
| Completeness [%] | 98.0 | |
| Redundancy | 2.8 | |
| CC(1/2) | 1.000 | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20 mM Tris-HCl, pH 7.9, 150 mM NaCl, 5mM MgCl2, 5 mM ATP, 1 mM sodium 2-mercaptoethanesulfonate |
