7COD

Post insertion complex of DNA polymerase Mu (K438A/Q441A) with 1-nt gapped DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	95
Detector technology	CCD
Collection date	2019-12-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	60.180, 68.520, 111.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.773 - 1.800
R-factor	0.1868
Rwork	0.186
R-free	0.21090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.773	29.773	1.850
High resolution limit [Å]	1.800	8.050	1.800
Rmerge	0.036	0.017	0.303
Rmeas	0.040	0.020	0.383
Total number of observations	195058
Number of reflections	41495	546	2264
<I/σ(I)>	24.19	60.16	2.55
Completeness [%]	95.1	96.1	71.5
Redundancy	4.701	4.359	2.223
CC(1/2)	1.000	0.999	0.893

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	298	1,4-Dioxane, Tris-Hcl, PEG 3350