7COB

Ternary complex of DNA polymerase Mu (Q441A) with 1-nt gapped DNA (T:dGMPNPP)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	95
Detector technology	CCD
Collection date	2019-10-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	59.760, 68.340, 110.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.663 - 1.797
R-factor	0.1809
Rwork	0.180
R-free	0.20380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.663	29.663	1.840
High resolution limit [Å]	1.797	8.040	1.800
Rmerge	0.049	0.026	0.576
Rmeas	0.055	0.030	0.637
Number of reflections	42776	539	3037
<I/σ(I)>	16.91	38.7	2.63
Completeness [%]	99.7	96.2	97.1
Redundancy	5.448	4.525	5.465
CC(1/2)	0.999	0.999	0.874

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	298	1,4-Dioxane, Tris-HCl, PEG 3350