7CO5
HtrA-type protease AlgW with decapeptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-09-20 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 95.120, 130.837, 250.364 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.500 - 2.345 |
R-factor | 0.2684 |
Rwork | 0.268 |
R-free | 0.28920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1soz |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.500 | 2.429 |
High resolution limit [Å] | 2.345 | 2.345 |
Number of reflections | 213255 | 11425 |
<I/σ(I)> | 2.96 | |
Completeness [%] | 96.1 | |
Redundancy | 3.4 | |
CC(1/2) | 0.796 | 0.262 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 290 | 2% Tacsimate PH 7.0, 5% 2-Propanol, 0.1M Imidazole PH 7.0, 8% PEG3350 |