7CNU
Crystal structure of DLC2 in complex with BMF peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-20 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 166.243, 38.701, 44.699 |
| Unit cell angles | 90.00, 99.87, 90.00 |
Refinement procedure
| Resolution | 41.930 - 2.000 |
| R-factor | 0.19645 |
| Rwork | 0.193 |
| R-free | 0.22565 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xqq |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.436 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.930 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 19215 | 1908 |
| <I/σ(I)> | 11.78 | |
| Completeness [%] | 99.0 | |
| Redundancy | 5.6 | |
| CC(1/2) | 0.997 | 0.951 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M Ammonium chloride 0.1 M HEPES 7.0 20 % w/v PEG 6000 |
