7CJX
UDP-glucuronosyltransferase 2B15 C-terminal domain-L446S
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-06-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.084, 142.035, 56.593 |
| Unit cell angles | 90.00, 115.42, 90.00 |
Refinement procedure
| Resolution | 51.115 - 1.986 |
| R-factor | 0.234160376972 |
| Rwork | 0.233 |
| R-free | 0.25879 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ipb |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.839 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | SHELXS |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.130 | 2.060 |
| High resolution limit [Å] | 1.980 | 1.990 |
| Rmeas | 0.133 | |
| Rpim | 0.051 | 0.051 |
| Number of reflections | 43041 | 43041 |
| <I/σ(I)> | 9.5 | |
| Completeness [%] | 87.6 | |
| Redundancy | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 18% PEG 6000, 0.2M Na-Tartrate, 0.2M KCl, 2% glycerol |
