7CII

Crystal structure of L-methionine decarboxylase from Streptomyces sp.590 in complexed with L- methionine methyl ester (external aldimine form).

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-25
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.00000
Spacegroup name	C 1 2 1
Unit cell lengths	125.322, 147.588, 54.011
Unit cell angles	90.00, 99.64, 90.00

Refinement procedure

Resolution	19.910 - 1.510
R-factor	0.17534
Rwork	0.174
R-free	0.19567
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7cif
RMSD bond length	0.010
RMSD bond angle	1.456
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.600
High resolution limit [Å]	1.510	1.510
Rmerge	0.094	0.714
Rpim	0.117	0.885
Number of reflections	143471	46587
<I/σ(I)>	6.5	1.3
Completeness [%]	97.9	96
Redundancy	2.6
CC(1/2)	0.992	0.623

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	0.1 M MMT buffer pH 6.0, 23.5 % (w/v) PEG1500