7CHY

Crystal Structure Of Human Il-1beta In Complex With Antibody Binding Fragment Of IgG26

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	98
Detector technology	CCD
Collection date	2015-12-13
Detector	RAYONIX MX300-HS
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	156.344, 112.240, 38.577
Unit cell angles	90.00, 94.85, 90.00

Refinement procedure

Resolution	19.700 - 2.650
Rwork	0.200
R-free	0.25350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2i1b
RMSD bond length	0.004
RMSD bond angle	1.264
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.763	2.740
High resolution limit [Å]	2.650	2.650
Number of reflections	19167	1911
<I/σ(I)>	20.7
Completeness [%]	100.0
Redundancy	2.7
CC(1/2)	0.760	0.760

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	3.8	293	17% (w/v) PEG 3350, 10% (v/v) glycerol, and 0.1 M citric acid at pH 3.8.