7CGA
Crystal structure of human unphosphorylated p38gamma
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 94 |
| Detector technology | CCD |
| Collection date | 2016-03-28 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 183.631, 183.631, 294.242 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.944 - 3.150 |
| Rwork | 0.160 |
| R-free | 0.21780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cm8 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.739 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.260 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rpim | 0.860 | |
| Number of reflections | 51419 | 5039 |
| <I/σ(I)> | 21.9 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.9 | 11 |
| CC(1/2) | 0.905 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 1.9 M Sodium Malonate |
