7CGA
Crystal structure of human unphosphorylated p38gamma
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL15A1 |
Synchrotron site | NSRRC |
Beamline | BL15A1 |
Temperature [K] | 94 |
Detector technology | CCD |
Collection date | 2016-03-28 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 1.00 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 183.631, 183.631, 294.242 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.944 - 3.150 |
Rwork | 0.160 |
R-free | 0.21780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1cm8 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.739 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.260 |
High resolution limit [Å] | 3.150 | 3.150 |
Rpim | 0.860 | |
Number of reflections | 51419 | 5039 |
<I/σ(I)> | 21.9 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 10.9 | 11 |
CC(1/2) | 0.905 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 1.9 M Sodium Malonate |