7CE9

PQQ-soaked Apo-methanol dehydrogenase (MDH) from Methylococcus capsulatus (Bath)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9
Spacegroup name	P 21 21 21
Unit cell lengths	128.563, 211.756, 223.736
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	153.000 - 2.200
R-factor	0.163
Rwork	0.161
R-free	0.20900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4tqo
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	153.790	2.330
High resolution limit [Å]	2.200	2.200
Number of reflections	292773	95297
<I/σ(I)>	11.76
Completeness [%]	99.2
Redundancy	3.3
CC(1/2)	0.900	0.900

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		291	Hepes, PEG600