7CDG

Crystal structure of LSD1-CoREST in complex with PRSFLVRRR peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-04
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.000
Spacegroup name	I 2 2 2
Unit cell lengths	122.246, 179.519, 233.338
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.000 - 2.800
R-factor	0.21206
Rwork	0.211
R-free	0.24204
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5h6q
RMSD bond length	0.002
RMSD bond angle	1.179
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.910	2.870
High resolution limit [Å]	2.800	2.800
Number of reflections	63360	4370
<I/σ(I)>	21.6	1.8
Completeness [%]	100.0
Redundancy	13.5
CC(1/2)	1.000	0.756

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M N-(carbamoylmethyl)iminodiacetic acid, 1.23 M potassium sodium tartrate tetrahydrate