7CDE

Crystal structure of LSD1-CoREST in complex with PRSFLVRKR peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-04
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.000
Spacegroup name	I 2 2 2
Unit cell lengths	121.597, 179.935, 232.632
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.000 - 2.680
R-factor	0.20782
Rwork	0.207
R-free	0.23301
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5h6q
RMSD bond length	0.004
RMSD bond angle	1.244
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.840	2.740
High resolution limit [Å]	2.680	2.680
Number of reflections	71734	4569
<I/σ(I)>	25.8	1.5
Completeness [%]	100.0
Redundancy	13.6
CC(1/2)	1.000	0.667

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M N-(carbamoylmethyl)iminodiacetic acid, 1.23 M potassium sodium tartrate tetrahydrate