7CD6

mAPE1-recessed dsDNA product complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2018-04-06
Detector	RAYONIX MX300-HS
Wavelength(s)	0.99984
Spacegroup name	P 63 2 2
Unit cell lengths	124.650, 124.650, 138.467
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.940 - 2.701
R-factor	0.1816
Rwork	0.178
R-free	0.21990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hd7
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	BALBES
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.800
High resolution limit [Å]	2.700	5.800	2.700
Rmerge	0.112	0.062	0.459
Rmeas	0.116	0.063	0.485
Rpim	0.026	0.013	0.151
Total number of observations	305693
Number of reflections	17975	1966	1733
<I/σ(I)>	7.4
Completeness [%]	99.9	99.7	99.6
Redundancy	17	22.4	8.9
CC(1/2)		0.999	0.865

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	0.1M Lithium sulfate monohydrate, 0.1M Na citrate tribasic dihydrate pH 5.5, 20% w/v Polyethylene glycol 1000