7CD2
Crystal structure of the S103F mutant of Bacillus subtilis (natto) YabJ protein.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2015-06-23 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 95.984, 96.121, 263.314 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.912 - 2.700 |
| R-factor | 0.19046 |
| Rwork | 0.186 |
| R-free | 0.27701 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5y6u |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.138 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 150.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.115 | 0.733 |
| Number of reflections | 62923 | 6046 |
| <I/σ(I)> | 15.5 | 3.1 |
| Completeness [%] | 93.3 | 91.3 |
| Redundancy | 11.8 | 11.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 293 | 25% PEG 4000, 0.45 M ammonium acetate, 0.1 M sodium acetate buffer pH 4.2 |
