7CCC

The structure of the actin filament uncapping complex mediated by twinfilin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2019-12-07
Detector	RAYONIX MX300HE
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	74.759, 168.662, 176.989
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.920 - 3.200
R-factor	0.1916
Rwork	0.186
R-free	0.23810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3daw 3aa7
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	20.000	3.050
High resolution limit [Å]	3.000	7.980	3.000
Rmerge	0.188	0.067	2.080
Rmeas	0.198	0.070	2.224
Rpim	0.061	0.021	0.761
Total number of observations	403423
Number of reflections	34562	2428	2097
<I/σ(I)>	4.5
Completeness [%]	95.7	99.1	94.4
Redundancy	9.3	9.6	7.2
CC(1/2)		0.997	0.495

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	12% PEG 8000, 10% glycerol, 500 mM potassium chloride